A business object can be used by declaring a local variable of the type of the business object, and subsequently, creating an instance of the business object using the NewInstance() static/shared method:

VB.NET:

Dim biz As ItemBusinessObject
biz = ItemBusinessObject.NewInstance()
biz.GetList()
biz.Dispose()

C#:

ProductBusinessObject biz = ProductBusinessObject.NewInstance();
biz.GetList();
biz.Dispose();